PCR
Data Input & Configuration Options
HELP: Parameters
- Max product size
- Min perfect match
- Min good match
- Flip reverse primer
Maximum size of amplified region.
Number of bases that match exactly on 3' end of primers. Minimum match size is 15.
Number of bases on 3' end of primers where at least 2 out of 3 bases match.
Invert the sequence order of the reverse primer and complement it.
Output sample
Amplified Sequences
Output |
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>chr13,32684164..32684358,(+),195bp,TTCTTCATCTGCTCTTTGTATTCCT|AATGGAAGCTCTGAGAGTCAAGAT TTCTTCATCTGCTCTTTGTATTCCTCAACATATGGAACAGCCAACTGCCTCAGCTGCTCC AGACGCTCAACAAGCTTAGCGCGCACAGACTCCACAATGGGCATCAGGGCGGCCTTGGTC TCCTCCACATTGGCGGCCATCTTCTGACGCAGCTCTGCCACCACGGGCTCCATCTTGACT CTCAGAGCTTCCATT |
Primer Melting Temperatures
Forward | 59.8 C TTCTTCATCTGCTCTTTGTATTCCT |
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Reverse | 59.9 C AATGGAAGCTCTGAGAGTCAAGAT |