Genome Tools


BLAST against Genome and Scaffold sequence

Parameters

Program blastn (DNA query vs. DNA database)
Database

Please specify the sequence data to reference.

File Upload
or COPY & PASTE
   

Options

Max target sequences
Expect threshold
Mask
   





HELP: BLAST Search main parameters

  • Expect

    • This setting specifies the statistical significance threshold for reporting matches against database sequences. The default value (10) means that 10 such matches are expected to be found merely by chance, according to the stochastic model of Karlin and Altschul (1990). If the statistical significance ascribed to a match is greater than the EXPECT threshold, the match will not be reported. Lower EXPECT thresholds are more stringent, leading to fewer chance matches being reported.